کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5533836 1550564 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Repression activity of Tailless on h 1 and eve 1 pair-rule stripes
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Repression activity of Tailless on h 1 and eve 1 pair-rule stripes
چکیده انگلیسی


- Several gap activities are necessary to set anterior pair-rule stripes boundaries.
- We tested whether Tailless (Tll) is part of this repression mechanism.
- Results: Stripes are disturbed in genetic assays involving Tll.
- Results: Tll misexpression confirmed tll repression on pair-rule stripes.
- Conclusion: Tll sets pair-rule stripes h 1 and eve 1.

We investigated the hypothesis that several transcriptional repressors are necessary to set the boundaries of anterior pair-rule stripes in Drosophila. Specifically, we tested whether Tailless (Tll) is part of a repression mechanism that correctly sets the anterior boundaries of hairy 1 (h 1) and even-skipped 1 (eve 1) stripes. Single mutant tll embryos displayed subtle deviations from the normal positions of h 1 and eve 1 stripes. Moreover, we observed stronger stripe deviations in embryos lacking both Tll and Sloppy-paired 1 (Slp 1), a common repressor for anterior pair-rule stripes. Using h 1 and eve 1 reporter constructs in the genetic assays, we provided further evidence that interference with normal mechanisms of stripe expression is mediated by Tll repression. Indeed, Tll represses both h 1 and eve 1 reporter stripes when misexpressed. Investigating the expression of other anterior gap genes in different genetic backgrounds and in the misexpression assays strengthened Tll direct repression in the regulation of h 1 and eve 1. Our results are consistent with tll being a newly-identified component of a combinatorial network of repressor genes that control pair-rule stripe formation in the anterior blastoderm of Drosophila.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mechanisms of Development - Volume 144, Part B, April 2017, Pages 156-162
نویسندگان
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