کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5534028 1550827 2017 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Exposure of decidualized HIESC to low oxygen tension and leucine deprivation results in increased IGFBP-1 phosphorylation and reduced IGF-I bioactivity
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Exposure of decidualized HIESC to low oxygen tension and leucine deprivation results in increased IGFBP-1 phosphorylation and reduced IGF-I bioactivity
چکیده انگلیسی


- Hypoxia/leucine deprivation modulate IGFBP-1 phosphorylation in decidualized HIESC.
- Hypoxia markedly increases IGFBP-1 phosphorylation at Ser169/Ser174 dual sites.
- IGFBP-1 phosphoisoforms are predominantly localized in the cytosol of HIESC.
- IGFBP-1 hyperphosphorylation reduces IGF-1 signaling at maternal/fetal interface.
- Reduced IGF-I signaling links phosIGFBP-1 to FGR during placental insufficiency.

Phosphorylation of decidual IGFBP-1 enhances binding of IGF-I, limiting the bioavailability of this growth factor which may contribute to reduced placental and fetal growth. The mechanisms regulating decidual IGFBP-1 phosphorylation are incompletely understood. Using decidualized human immortalized endometrial stromal cells we tested the hypothesis that low oxygen tension or reduced leucine availability, believed to be common in placental insufficiency, increase the phosphorylation of decidual IGFBP-1. Multiple reaction monitoring-MS (MRM-MS) was used to quantify IGFBP-1 phosphorylation. MRM-MS validated the novel phosphorylation of IGFBP-1 at Ser58, however this site was unaffected by low oxygen tension/leucine deprivation. In contrast, significantly elevated phosphorylation was detected for pSer119, pSer98/pSer101 and pSer169/pSer174 sites. Immunoblotting and dual-immunofluorescence using phosphosite-specific IGFBP-1 antibodies further demonstrated increased IGFBP-1 phosphorylation in HIESC under both treatments which concomitantly reduced IGF-I bioactivity. These data support the hypothesis that down regulation of IGF-I signaling links decidual IGFBP-1 hyperphosphorylation to restricted fetal growth in placental insufficiency.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Endocrinology - Volume 452, 5 September 2017, Pages 1-14
نویسندگان
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