کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5534235 | 1550838 | 2017 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Role of cAMP/PKA pathway and T-type calcium channels in the mechanism of action of serotonin in human adrenocortical cells Role of cAMP/PKA pathway and T-type calcium channels in the mechanism of action of serotonin in human adrenocortical cells](/preview/png/5534235.png)
- Serotonin (5-HT) is produced by mast cells located in zona glomerulosa of human adrenal.
- 5-HT receptor type 4 (5-HT4) is expressed by cortisol-producing cells positive for 11β-hydroxylase.
- 5-HT4 receptors are positively coupled to adenylyl cyclase (AC) and cAMP-dependent protein kinase (PKA).
- Activation of PKA stimulates Ca2+ influx through T- and L-type Ca2+ channels, and cortisol production.
In human adrenal, serotonin (5-HT), produced by mast cells located in zona glomerulosa, stimulates production of corticosteroids through a paracrine mechanism involving the 5-HT receptor type 4 (5-HT4). The aim of the present study was to investigate the transduction mechanisms associated with activation of 5-HT4 receptors in human adrenocortical cells. Our results show that 5-HT4 receptors are present in the outer adrenal cortex, both in glomerulosa and fasciculata zonae. In the zona glomerulosa. 5-HT4 receptor was detected both in immunopositive and immunonegative cells for 11β-hydroxylase, an enzyme involved in cortisol synthesis. The data demonstrate that 5-HT4 receptors are positively coupled to adenylyl cyclases and cAMP-dependent protein kinases (PKA). The activation of the cAMP-PKA pathway is associated with calcium influx through T-type calcium channels. Both the adenylyl cyclase/PKA pathway and the calcium influx are involved in 5-HT-induced cortisol secretion.
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Journal: Molecular and Cellular Endocrinology - Volume 441, 5 February 2017, Pages 99-107