کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5534533 1551169 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Assessment of NS1 gene-specific real time quantitative TaqMan PCR for the detection of Human Bocavirus in respiratory samples
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Assessment of NS1 gene-specific real time quantitative TaqMan PCR for the detection of Human Bocavirus in respiratory samples
چکیده انگلیسی
Human Bocaviruses (HBoV) were associated with respiratory diseases. Here, we assessed a TaqMan®-based PCR for the detection of all four HBoV subtype infections with a sensitivity up to 15 copies/reaction. To evaluate this assay on clinical samples, 178 nasopharyngeal aspirate specimens from pediatric cases were analyzed and HBoV genome was detected in 13 out of 178 patients with a viral load range between 1.6 × 103 and 9.4 × 107 copies/ml. These results indicated that this method could be used as an alternative technique for the diagnosis of HBoV infection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Probes - Volume 34, August 2017, Pages 53-55
نویسندگان
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