کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5534609 | 1551270 | 2017 | 10 صفحه PDF | دانلود رایگان |
- Capillarisin activates Nrf2/ARE/HO-1 signaling in neural and microglial cells.
- JNK activation is responsible for Nrf2 phosphorylation.
- Capillarisin protects SH-SY5Y neuronal cells from 6-OHDA-induced oxidative damage.
- Capillarisin inhibits LPS-induced inflammatory responses in BV2 microglial cells.
- Capillarisin may combat oxidative stress and inflammatory responses in the brain.
Capillarisin is a naturally isolated chromone, which is one of the major bioactive constituents of Artemisia capillaries. Capillarisin has antioxidant, anti-inflammatory, and anti-tumor potential, but the underlying molecular mechanisms remain largely unclear. In the present study, we demonstrate that the transcription factor nuclear factor E2-related factor-2 (Nrf2) is activated by capillarisin in neuroblastoma SH-SY5Y cells and microglial BV2 cells. Capillarisin leads to Nrf2 phosphorylation, subsequent activation of antioxidant response element (ARE)-mediated transcription, and up-regulation of downstream molecules, such as heme oxygenase-1 (HO-1) and NAD(P)H:quinone oxidoreductase 1. Capillarisin protects SH-SY5Y cells from 6-hydroxydopamine-induced oxidative stress and attenuates inflammatory responses in lipopolysaccharide-treated BV2 cells. The cytoprotective and anti-inflammatory effects of capillarisin are significantly abolished in cells transfected with specific Nrf2 or HO-1 siRNA, suggesting that these pharmacological properties of capillarisin are primarily due to increased HO-1 activity. Capillarisin induces the activation of c-Jun N-terminal kinase in SH-SY5Y and BV2 cells, which is responsible for Nrf2 phosphorylation and HO-1 upregulation. Together, this study demonstrates that capillarisin is a potential activator of the Nrf2/ARE-dependent pathway and could be an attractive candidate for the regulation of oxidative stress and inflammatory responses in the brain.
Journal: Neurochemistry International - Volume 105, May 2017, Pages 11-20