Colorimetric detection of caprine arthritis encephalitis virus (CAEV) through loop-mediated isothermal amplification (LAMP) with gold nanoprobes

- CAE is economically important disease of goats that widely affect their productivity.
- Molecular technique such as LAMP has been applied to detect the CAEV.
- Oligonucleotide or probe was designed and Gold nanoparticles was synthesized to generate a Gold nanoprobe (AuPr).
- Gold nanoprobe (AuPr) was used for colorimetric detection of CAEV LAMP product.
- An excellent but simple method to detect CAEV in LAMP product using AuPr.

Infectious diseases in goats, CAE in particular have widely affected the productivity of this animal and greatly affected the farmers and the small ruminant industry. Molecular technique such as LAMP has been applied to detect the CAEV proviral DNA specifically and sensitively but this technique has its own drawback. Gold is the most widely used nanoparticle (NP) which has excellent properties such as high surface area and compatibility with biomolecules. In this study, gold nanoparticle (AuNP) conjugated with modified oligonucleotides or gold nanoprobe (AuPr) was used to detect CAEV proviral DNA in LAMP product. The hybridization of AuPr to a complementary sequence on the LAMP product made the gold resistant to high salt concentration. This resistance or non-resistance of the AuNP is observed as color change in the mixture. This study, reported a simple method of visual detection of CAEV proviral DNA in LAMP product using AuPr.