کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5587749 1568861 2017 45 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Testosterone and progesterone concentrations in blow samples are biologically relevant in belugas (Delphinapterus leucas)
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی علوم غدد
پیش نمایش صفحه اول مقاله
Testosterone and progesterone concentrations in blow samples are biologically relevant in belugas (Delphinapterus leucas)
چکیده انگلیسی
Steroid hormone analysis in blow (respiratory vapor) may provide a minimally invasive way to assess the reproductive status of wild cetaceans. Biological validation of the method is needed to allow for the interpretation of hormone measurements in blow samples. Utilizing samples collected from trained belugas (Delphinapterus leucas, n = 20), enzyme immunoassays for testosterone and progesterone were validated for use with beluga blow samples. Testosterone concentrations in 40 matched blood and blow samples collected from 4 male belugas demonstrated a positive correlation (R2 = 0.52, p < 0.0001). Progesterone concentrations in 64 matching blood and blow samples from 11 females were also positively correlated (R2 = 0.60, p < 0.0001). Testosterone concentrations (mean ± SD) in blow samples collected from adult males (119.3 ± 14.2 pg/ml) were higher (p < 0.01) than that of a juvenile male (<8 years) (59.4 ± 6.5 pg/ml) or female belugas (54.1 ± 25.7 pg/ml). Among adult males, testosterone concentrations in blow demonstrated a seasonal pattern of secretion, with peak secretion occurring during the breeding season (February-April, 136.95 ± 33.8 pg/ml). Progesterone concentrations in blow varied by reproductive status; pregnant females (410.6 ± 87.8 pg/ml) and females in the luteal phase of the estrous cycle (339.5 ± 51.0 pg/ml) had higher (p < 0.0001) blow progesterone concentrations than non-pregnant females without a corpus luteum (242.5 ± 27.3 pg/ml). Results indicate that blow sample analysis can be used to detect variation in reproductive states associated with large differences in circulating testosterone or progesterone in belugas.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: General and Comparative Endocrinology - Volume 246, 15 May 2017, Pages 183-193
نویسندگان
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