کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589328 | 1569819 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Sp3 controls fibroblast growth factor receptor 4 gene activity during myogenic differentiation
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کلمات کلیدی
TSSGCFAP2FGFR4MyogenesisActivator protein 2Differentiation mediachromatin immunoprecipitation - ایمن سازی کروماتینProliferation - ترویجDifferentiation - تفکیکbase pairs - جفت پایهGrowth media - رسانه های رشدtranscription start site - رونویسی شروع سایتCHiP - چیپfibroblast growth factor receptor 4 - گیرنده فاکتور رشد فیبروبلاست 4
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Fibroblast growth factor/fibroblast growth factor receptor (FGF/FGFR) signaling is a critical component in the regulation of myoblast proliferation and differentiation. The transient FGFR4 gene expression during the transition from proliferating myoblasts to differentiated myotubes indicates that FGFR4 regulates this critical phase of myogenesis. The Specificity Protein (SP) family of transcription factors controls FGFR family member gene activity. We sought to determine if members of the Sp family regulate mouse FGFR4 gene activity during myogenic differentiation. RT-PCR and western blot analysis of FGFR4 mRNA and protein revealed transient expression over 72Â h, with peak expression between 24 and 36Â h after addition of differentiation medium to C2C12 myogenic cultures. Sp3 also displayed a transient expression pattern with peak expression occurring after 6Â h of differentiation. We cloned a 1527Â bp fragment of the mouse FGFR4 promoter into a luciferase reporter. This FGFR4 promoter contains eight putative Sp binding sites and directed luciferase gene activity comparable to native FGFR4 expression. Overexpression of Sp1 and Sp3 showed that Sp1 repressed FGFR4 gene activity, and Sp3 activated FGFR4 gene activity during myogenic differentiation. Mutational analyses of multiple Sp binding sites within the FGFR4 promoter revealed that three of these sites were transcriptionally active. Electromobility shift assays and chromatin immunoprecipitation of the area containing the activator sites showed that Sp3 bound to this promoter location.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 617, 20 June 2017, Pages 24-31
Journal: Gene - Volume 617, 20 June 2017, Pages 24-31
نویسندگان
Eric Cavanaugh, Joseph X. DiMario,