کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5589461 1569839 2017 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Distinct gene networks drive differential response to abrupt or gradual water deficit in potato
ترجمه فارسی عنوان
شبکه های ژن های واجد شرایط پاسخ دیفرانسیل را به کمبود شدید یا کمبود آب در سیب زمینی هدایت می کنند
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
چکیده انگلیسی
To validate microarray data obtained for potato cells, the expression analysis of 21 selected genes of interest was performed by Real-Time Quantitative Reverse Transcription PCR (qRT-PCR). Intriguingly, the expression levels of these transcripts in 4-week old potato plants exposed to long-term water-deficit. qRT-PCR analysis showed that several genes were regulated similarly in potato cells cultures and tissues exposed to drought, thus confirming the efficacy of our simple experimental system to capture important genes involved in osmotic stress response. Highlighting the differences in gene expression between shock-like and adaptive response, our findings could contribute to the discussion on the biological function of distinct gene networks involved in the response to abrupt and gradual adaptation to water deficit.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 597, 15 January 2017, Pages 30-39
نویسندگان
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