کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589610 | 1569809 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel GhBEE1-Like gene of cotton causes anther indehiscence in transgenic Arabidopsis under uncontrolled transcription level
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کلمات کلیدی
BRS24-epi-brassinolideColumbia-0bHLHDPAGFPArabidopsis - آرابیدوپسیس یا رشادیbasic helix-loop-helix - اسلحه پایه حلقه ایjasmonic acid - اسید جاسونیکAnther dehiscence - انهدام انترنتیBrassinosteroid - براسین استروئیدBrassinosteroids - برسینستروئید هاdays post-anthesis - روز پس از انتشاریgreen fluorescent protein - پروتئین فلورسنت سبزCotton - کتانCol-0 - کل 0Gas - گازGibberellins - گیببرلینز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Male-sterile lines are very important for selective breeding, and anther dehiscence defect is an effective way to generate male-sterile lines. Although several bHLH-family proteins in Arabidopsis have been characterized, little is known about the role of bHLH-family proteins in cotton. Here, we isolated a novel bHLH protein from cotton (Gossypium hirsutum), named GhBEE1-Like. Protein domain analysis showed that GhBEE1-Like contained a basic domain and an HLH domain. Subcellular localization analysis revealed that GhBEE1-Like was a nuclear-localized protein. Expression pattern analysis showed GhBEE1-Like was highly expressed in floral organs, and its expression was induced by the active brassinosteroid (BR) substance 24-epi-BL. GhBEE1-Like overexpression in Arabidopsis resulted in two types of transgenic lines, one with normal anther dehiscence and the other with defective anther dehiscence. Semi-qRT-PCR and qRT-PCR analyses revealed that GhBEE1-Like transcript levels acted as a check-point determining how anther dehiscence proceeds in these transgenic lines; regulated transcript levels result in normal anther dehiscence, whereas uncontrolled transcript levels lead to anther indehiscence. These results suggest that GhBEE1-Like plays an important role via its accumulation in regulating anther dehiscence. Therefore, controlling the level of GhBEE1-Like expression in cotton could be a convenient tool for generating male-sterile lines to use in selective breeding.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 627, 5 September 2017, Pages 49-56
Journal: Gene - Volume 627, 5 September 2017, Pages 49-56
نویسندگان
Eryong Chen, Xiaoqian Wang, Qian Gong, Hamama Islam Butt, Yanli Chen, Chaojun Zhang, Zuoren Yang, Zhixia Wu, Xiaoyang Ge, Xianlong Zhang, Fuguang Li, Xueyan Zhang,