کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589678 | 1569823 | 2017 | 34 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification and characterization of a novel multifunctional placenta specific protein 8 in Dugesia japonica
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کلمات کلیدی
LPSNCBIORFGFPPlanarianrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدDevelopment - رشدPharynx - فریونکسopen reading frame - قاب خواندن بازlipopolysaccharide - لیپوپلی ساکاریدNational Center for Biotechnology Information - مرکز ملی اطلاعات بیوتکنولوژیRace - مسابقهImmunity - مصونیتUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهgreen fluorescent protein - پروتئین فلورسنت سبز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
Placenta specific protein 8 (Plac8) has been well studied in vertebrates, yet little is known in invertebrates. In this study, a novel Plac8 from the planarian Dugesia japonica was identified and its functions in immune responses and development were characterized. Our results show that Djplac8 was expressed in the pharynx, epidermis and intestine of intact adult planarian. The expression of DjPlac8 increased significantly upon lipopolysaccharide (LPS) challenge, and inhibited the growth of the Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), suggesting the role of Plac8 in immune response. Spatial and temporal expression and distribution of DjPlac8 mRNA in regenerated planarians indicates that DjPlac8 was mainly expressed in the pharynx. In situ hybridization also revealed the elevated expression of the DjPlac8 gene in the embryonic pharynx, germ band and parenchyma cells, indicating an important role in embryonic development of D. japonica. When DjPlac8 was deactivated by RNA interference-mediated knockdown, the head of planarians underwent abnormal development. In summary, we identified DjPlac8 as a novel multifunctional protein that plays essential roles in immune response and development of planarians.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 613, 20 May 2017, Pages 1-9
Journal: Gene - Volume 613, 20 May 2017, Pages 1-9
نویسندگان
Qiuxiang Pang, Lili Gao, Yun Bai, Hongkuan Deng, Yu Han, Wenjing Hu, Yanhui Zhang, Shanhang Yuan, Wenping Sun, Yiqian Lu, Xiufang Zhang, Baohua Liu, Bosheng Zhao,