کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589798 | 1569821 | 2017 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A putative 12-oxophytodienoate reductase gene CsOPR3 from Camellia sinensis, is involved in wound and herbivore infestation responses
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کلمات کلیدی
OPRORFAOCOPDA12-oxo-phytodienoic acid - 12-oxo-phytandienoic acidallene oxide cyclase - آلکالن اکسید سیکلاسα-linolenic acid - اسید α-لینولنیکjasmonic acid - اسید جاسونیکLOX - اکسیژن مایعAOS - بهrapid-amplification of cDNA ends - تکثیر سریع cDNA به پایان می رسدrelative air humidity - رطوبت نسبی هواWounding - زخمیAllene oxide synthase - سنتاز اکسید آلنopen reading frame - قاب خواندن بازlipoxygenase - لیپواکسیژنازRace - مسابقهTEA - چای
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
12-Oxophytodienoate reductase (OPR) is a key enzyme in the biosynthesis of jasmonic acid (JA), which plays an important role in plant defense responses. Although multiple isoforms of OPRs have been identified in various annual herbaceous plants, genes encoding these enzymes in perennial woody plants have yet to be fully investigated. In the tea plant, Camellia sinensis (L.), no OPR genes have been isolated, and their possible roles in tea plant development and defense mechanism remain unknown. In this study, a putative OPR gene, designated as CsOPR3, was isolated from tea plants for the first time through the rapid amplification of cDNA ends. The open reading frame of CsOPR3 is 1197Â bp in length, and encodes a protein of 398 amino acids. Real-time qPCR analysis revealed that CsOPR3 was expressed in different organs. In particular, CsOPR3 was highly expressed in flowers, leaves and stems but was weakly expressed in roots and seeds. CsOPR3 expression could be rapidly induced by mechanical wounding, and increased JA levels were correlated with the wound-induced CsOPR3 expression. The infestation of the tea geometrid (TG) Ectropis obliqua Prout, regurgitant derived from TG and exogenous JA application could enhance the CsOPR3 expression. Our study is the first to report that CsOPR3 plays an important role in JA biosynthesis and tea plant defense against herbivorous insects.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 615, 5 June 2017, Pages 18-24
Journal: Gene - Volume 615, 5 June 2017, Pages 18-24
نویسندگان
Zhaojun Xin, Jin Zhang, Lingang Ge, Shu Lei, Juanjuan Han, Xin Zhang, Xiwang Li, Xiaoling Sun,