کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5590210 | 1570115 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Traditional versus 3â² RNA-seq in a non-model species
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
One limitation of the widely used RNA-seq method is that long transcripts are represented by more reads than shorter transcripts, resulting in a biased estimation of expression levels. The 3â² RNA-seq method, which yields only one sequence per transcript, bypasses this limitation. Here, RNA was extracted from two samples, in which we expected to find differentially expressed genes. Each was processed by both traditional and 3â² RNA-seq protocols. Both methods yielded similar differentially expressed genes and estimated expression levels in a comparable way, confirming they both represent valid tools for RNA-seq analysis. Notably, however, we identified more differentially expressed transcripts with the 3â² RNA-seq method, suggesting a greater power to detect expression variation using this method. Hence, when little genomic information is available for the species studied, the standard RNA-seq presents a better cost-benefit compromise, whereas for model species, the 3â² RNA-seq method might more accurately detect differential expression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Genomics Data - Volume 11, March 2017, Pages 9-16
Journal: Genomics Data - Volume 11, March 2017, Pages 9-16
نویسندگان
Sophie Tandonnet, Tatiana Teixeira Torres,