کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5591878 | 1570703 | 2017 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
LEI0258 microsatellite variability and its association with humoral and cell mediated immune responses in broiler chickens
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
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![عکس صفحه اول مقاله: LEI0258 microsatellite variability and its association with humoral and cell mediated immune responses in broiler chickens LEI0258 microsatellite variability and its association with humoral and cell mediated immune responses in broiler chickens](/preview/png/5591878.png)
چکیده انگلیسی
Major histocompatibility complex (MHC) has a profound influence on disease resistance or susceptibility, productivity and important economic traits in chicken. Association of the MHC with a wide range of immune responses makes it a valuable predictive factor for the disease pathogenesis and outcome. The tandem repeat LEI0258 is a genetic marker which is located within the B locus of chicken MHC and strongly associated with serologically defined haplotypes. LEI0258 microsatellite marker was applied to investigate the MHC polymorphism in Ross 308 broiler chicken (NÂ =Â 104). Association of LEI0258 alleles with humoral and cell mediated immune responses to Newcastle disease (ND), Infectious bursal disease (IBD) and Avian influenza (AI) vaccines were also examined. LEI0258 polymorphism was determined by PCR-based fragment analysis, and association of LEI0258 alleles with immune responses were evaluated using multivariate regression analysis and GLM procedures. A total of seven alleles ranging from 195 to 448Â bp were found, including two novel alleles (263 and 362Â bp) that were unique in Ross 308 broiler population. Association study revealed a significant influence of MHC alleles on humoral and cellular immune responses in Ross population (PÂ <Â 0.05). Alleles 385 and 448Â bp were associated with increased peripheral blood lymphocyte proliferation response. Alleles 300, 362 and 448Â bp had a positive effect on immune responses to Infectious bursal disease vaccine, and allele 263Â bp was significantly correlated with elevated antibody titer against Newcastle disease vaccine. Results obtained from this study confirmed the important role of MHC as a candidate gene marker for immune responses that could be used in genetic improvement of disease-resistant traits and resource conservation in broiler population.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 90, October 2017, Pages 22-26
Journal: Molecular Immunology - Volume 90, October 2017, Pages 22-26
نویسندگان
Atefeh Esmailnejad, Gholamreza Nikbakht Brujeni, Maryam Badavam,