کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5593418 | 1571080 | 2017 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Bama miniature pigs' liver possess great heat tolerance through upregulation of Nrf2-mediated antioxidative enzymes
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
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![عکس صفحه اول مقاله: Bama miniature pigs' liver possess great heat tolerance through upregulation of Nrf2-mediated antioxidative enzymes Bama miniature pigs' liver possess great heat tolerance through upregulation of Nrf2-mediated antioxidative enzymes](/preview/png/5593418.png)
چکیده انگلیسی
The liver is one of the most crucial organs affected by high ambient temperature. Bama miniature pig show a heat tolerance in hot summer months. However, the physiological condition of liver under high ambient temperature has not been well elucidated in Bama miniature pig. Here we performed an experiment to investigate the effects of high ambient temperature on liver function, redox status and Nrf2 antioxidant pathway in Bama miniature pigs. Twelve pigs were randomly divided into two groups and separately exposed to the neutral temperature (NT, 25 °C) and high temperature (HT, 40 °C) for 8 days. The hepatic damage marker, such as total bilirubin (TBIL), alkaline phosphatase (AKP), γ-glutamyl transpeptidas (γ-GT), alanine transaminase (ALT) and aspartase transminase (AST), didn't reach statistical significance between NT and HT group. Moreover, abnormal observation of hepatic histology and hepatocyte ultrastructure were not detected in HT group. The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) as well as glutathione (GSH) content, were dramatically increased after heat exposure. Heat treatment didn't increase hydrogen peroxide (H2O2) and malondialdehyde (MDA) concentrations. The expression of Nrf2-regulated genes, such as nuclear factor erythroid 2-related factor 2 (Nrf2), NAD(P)H: quinine oxidoreductase 1 (NQO1), superoxide dismutase 1 (SOD1), heme oxygenase-1 (HO-1) and glutamate cysteine ligase catalytic subunit (GCLC), were significantly increased in HT group. Nrf2 protein was accumulated in HT group through immunohistochemical analysis. The current data provide clear evidence that Bama miniature pigs' liver possess great capacity of heat tolerance, which related to activation of Nrf2 antioxidant pathway.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Thermal Biology - Volume 67, July 2017, Pages 15-21
Journal: Journal of Thermal Biology - Volume 67, July 2017, Pages 15-21
نویسندگان
Jiaqin Chen, Fei Wang, Xin Zhou, Yun Cao, Yansen Li, Chunmei Li,