کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5736315 1613223 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research reportInhibition of platelet-derived growth factor receptor β reduces reactive glia and scar formation after traumatic brain injury in mice
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
Research reportInhibition of platelet-derived growth factor receptor β reduces reactive glia and scar formation after traumatic brain injury in mice
چکیده انگلیسی


- PDGFRβ was activated in mice after traumatic brain injury (TBI).
- Activated PDGFRβ was primarily expressed in astrocytes, microglia, OPC and leukocytes.
- PDGFRβ inhibitor (AG1296) reduced reactive glia in TBI mice.
- AG1296-treatment disrupted scar formation after TBI.

Brain injury leads to complex cellular and molecular interactions within the central nervous system. As the glial scar was a mechanical barrier to regeneration, inhibitory molecules in the forming scar and methods to overcome them have suggested molecular modification strategies to allow neuronal growth and functional regeneration. Here we investigated the roles of PDGFRβ signaling in regulating astrocyte reactivity and scar formation in mice following traumatic brain injury (TBI). The expression and distribution of phosphorylated PDGFRβ was analyzed, and its cell type-specific expression was verified with double labeling of astrocytes (GFAP), microglia (IBA1), oligodendrocyte precursor cells (OPC) (NG2) and leukocytes (CD45). We found PDGFRβ was activated around the injury site after TBI, and primarily expressed in astrocytes, microglia, OPC and leukocytes in the boundary of the lesion site, suggesting PDGFRβ was involved in glial scar formation. Then the PDGFR inhibitor (AG1296) was administered following TBI. Reactive astrocytes were significantly inhibited in AG1296-treated mice. Furthermore, AG1296-treatment attenuated reactive leukocytes, OPC and astrocytes and pronouncedly disrupted of glial scar formation after TBI. These findings prove that PDGFRβ signaling inhibited reactive glia-mediated scar formation after TBI in mice.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research Bulletin - Volume 134, September 2017, Pages 121-127
نویسندگان
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