کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5736396 1613225 2017 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Establishment and characterization of primary astrocyte culture from adult mouse brain
ترجمه فارسی عنوان
ایجاد و تعریف فرهنگ آستروسیت اولیه از مغز موش بالغ
کلمات کلیدی
آستروسیت، فرهنگ اولیه، بزرگسالان، ماوس، اموال بیولوژیک، تابع،
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب سلولی و مولکولی
چکیده انگلیسی
As a major class of glial cells, astrocytes have been indicated to play multi-roles in physiological and pathological brain. Astrocyte cultures derived from postnatal mouse brains have been extensively used to characterize their biological properties. However, the inability to culture adult mouse primary astrocytes has long stymied studies of function in adult brain. Here, we developed a protocol to successfully establish highly enriched astrocyte cultures from the brains of adult mouse. Cortical tissues were collected to prepare cell suspension by enzymatic digestion and mechanical dissociation, and then plated onto vessels pre-coated with gelatin and matrigel and cultured in DMEM medium containing 20% fetal bovine serum (FBS). Forskolin (FSK) and glial-derived neurotrophic factor (GDNF) were use to promote astrocyte proliferation and survival respectively. These adult astrocyte cultures were identified by immunocytochemical, immunobloting and PCR analysis. Furthermore, biological and functional analysis indicated that they possess the biochemical and physiological properties of astrocytes, suggestive of a useful cell model for astroglial studies in adult brain.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research Bulletin - Volume 132, June 2017, Pages 10-19
نویسندگان
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