کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5737229 1614580 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research PaperVisualization of migration of human cortical neurons generated from induced pluripotent stem cells
ترجمه فارسی عنوان
مقاله پژوهشی بررسی سیستم مهاجرت نورونهای قشر انسانی تولید شده از سلول های بنیادی پلورپوفتون القا شده
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
چکیده انگلیسی


- We developed the new methods to observe the migratory behavior of the human cortical neuron derived from induced pluripotent stem cells in vitro.
- Cortical neurons derived from induced pluripotent stem cells showed two-stroke like movement.
- We successfully generated migrating cortical neurons from lissencephaly-patient derived induced pluripotent stem cells.

BackgroundNeuronal migration is considered a key process in human brain development. However, direct observation of migrating human cortical neurons in the fetal brain is accompanied by ethical concerns and is a major obstacle in investigating human cortical neuronal migration.New methodWe established a novel system that enables direct visualization of migrating cortical neurons generated from human induced pluripotent stem cells (hiPSCs).ResultsWe observed the migration of cortical neurons generated from hiPSCs derived from a control and from a patient with lissencephaly.MethodsOur system needs no viable brain tissue, which is usually used in slice culture. Migratory behavior of human cortical neuron can be observed more easily and more vividly by its fluorescence and glial scaffold than that by earlier methods.ConclusionsOur in vitro experimental system provides a new platform for investigating development of the human central nervous system and brain malformation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Neuroscience Methods - Volume 289, 1 September 2017, Pages 57-63
نویسندگان
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