کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5739543 | 1615837 | 2017 | 9 صفحه PDF | دانلود رایگان |
- Preservation methods were tested in eight species of entomopathogenic fungi (EPF).
- The phenotypic characteristics were evaluated qualitatively after recovery.
- The EPF species showed differences depending of the preservation method.
- AFLP study did not show genomic instability in the eight EPF species after recovery.
- Cryopreservation at â196 °C was proposed as the best method to preserve EPF species.
Preservation methods for entomopathogenic fungi (EPF) require effective protocols to ensure uniform processes and to avoid alterations during storage. The aim of this study was to preserve Beauveria bassiana, Metarhizium acridum, M. anisopliae, M. rileyi, Isaria javanica, Hirsutella thompsonii, H. citriformis and Lecanicillium lecanii in mineral oil (MO), sterile water (SW), silica gel (SG), lyophilisation (L), ultracold-freezing at â70 °C, and cryopreservation at â196 °C. The viability and purity of the fungi were then verified: phenotypic characteristics were evaluated qualitatively at 6, 12 and 24 m. Genetic stability was tested by amplified fragment length polymorphisms (AFLP) analysis at 24 m. Of the eight species of EPF, three remained viable in SW, five in MO and L, six at â70 °C, seven in SG, and eight at â196 °C. No significant changes were observed in AFLP patterns at 24 m of storage. The most effective preservation methods for EPF were SG, L, â70 and â196 °C. Beauveria bassiana, M. acridum, M. anisopliae, M. rileyi and I. javanica remained stable with all methods, while the remaining species were less compatible. The optimisation of preservation methods for EPF facilitates the development of reliable protocols to ensure their inherent characteristics in culture collections.
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Journal: Fungal Biology - Volume 121, Issue 11, November 2017, Pages 920-928