کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5740111 | 1616233 | 2017 | 7 صفحه PDF | دانلود رایگان |
- E. durans 152 showed anti-listerial activity in both broth culture and floor drain environments.
- The bacteriocins produced by E. durans 152 were characterized.
- Partially purified bacteriocins of Dur 152 inhibited the growth of Listeria in ham.
- The anti-listerial activity of Dur 152 was better than Nisin.
Listeria monocytogenes is a nonfastidious, widely occurring foodborne pathogen that is a major challenge to the food industry. Enterococcus durans 152, a confirmed L. monocytogenes-control microorganism, was isolated from floor drain samples from a food processing facility. In this study, the two bacteriocins produced by E. durans 152 were characterized and identified as Dur 152A (an enterocin L50A derivative with two amino acid substitutions of IâM) and enterocin L50B. The bacteriocins were then partially purified and evaluated for inhibitory activity to L. monocytogenes in deli ham. Results revealed that at 400 AU/ml, the bacteriocins prevented growth of listeria in deli ham for at least 10 weeks at 8 °C and at least 30 days at 15 °C. For comparison, 500 ppm Nisin controlled listeria growth for up to 6 weeks at 8 °C and up to 18 days at 15 °C. These findings reveal the potential for the bacteriocins of E. durans 152 to serve as anti-listerial agents in deli meat.
Journal: Food Microbiology - Volume 68, December 2017, Pages 97-103