کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5763178 1625146 2017 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Asymbiotic germination and seed storage of Paphiopedilum insigne, an endangered lady's slipper orchid
ترجمه فارسی عنوان
جوانه زنی اسمبیبیوتیک و نگهداری دانه از پیکوفیدیلوم، یک ارکیده دمپایی بانوی خطرناک
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم زراعت و اصلاح نباتات
چکیده انگلیسی
The present scenario of urbanization and commercialization has adversely affected orchid's population; as a consequence they are diminishing from the nature very rapidly. Paphiopedilum insigne (lady's slipper orchid) is one such orchid of horticultural importance. Apart from being listed as endangered in IUCN red data list; it finds a place in Appendix I of CITES in the global platform. In the present study, capsules < 180 days after pollination (DAP) were found to be immature and 180 DAP was found optimal for seed germination. Capsules > 240 DAP were found to have mature seeds that were treated prior to germination and TTC tested for viability. Seeds derived from 180 DAP capsules showed the highest germination of 88.5% in modified Burgeff medium (BG1) with initiation in 26 days. Incorporation of plant growth regulators like 5 μM kinetin (KN) + 10 μM indole-3-acetic acid (IAA) in 1/2 MS medium influenced the stage-wise development of the seedlings in a short duration. Mature seeds stored at − 196 °C for 360 days followed by pre-treatment with 3% NaOCl showed viability of 70.5% and recorded germination of 79.2%. Sixty-nine percent of the plantlets were successfully hardened and acclimatized in the green house.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: South African Journal of Botany - Volume 112, September 2017, Pages 215-224
نویسندگان
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