Inheritance analysis of anthracnose resistance and marker-assisted selection in introgression populations of chili (Capsicum annuum L.)

Two Capsicum annuum anthracnose resistant introgression lines PR1 (derived from PBC932C. chinense) and PR2 (derived from PBC80C. baccatum) with resistance conferred by different sources were crossed to a susceptible parent (PS) and F1, F2, BC1PS, BC1PR and F2 of a three-way populations were generated. Fruits of the populations were inoculated with Colletotrichum acutatum-Ca153 using microinjection method at the mature green stage. The SCAR-Indel and SSR-HpmsE032 markers were used for validation in all populations derived from PR1 and PR2, respectively. In both resistance sources (PR1 and PR2), resistance was controlled by a major recessive gene. The resistant banding pattern amplified by SCAR-Indel and SSR-HpmsE032 markers was 100 bp and 231 bp fragments, respectively. Validation studies of both markers in F2 populations revealed that individually their ability to correctly predict resistant genotypes (selection efficiency) was 65%. However, when both markers were used together, the selection efficiency increased to 77% as well as the efficacy of individual SSR marker used within F2 of the three-way populations.