کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5769624 | 1628778 | 2017 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Carotenoid accumulation and gene expression during durian (Durio zibethinus) fruit growth and ripening Carotenoid accumulation and gene expression during durian (Durio zibethinus) fruit growth and ripening](/preview/png/5769624.png)
- The durian cultivar 'Chanee' had higher β-, α-carotene and lutein content thancv. 'Monthong'.
- 'Monthong' had higher minor zeaxanthin content than 'Chanee'.
- Total carotenoid content was 6-fold higher in ripe 'Chanee' than in ripe 'Monthong'.
- Accumulation of β-and α-carotenein durian is regulated by expression of the genes ZDS, LCYE and LCYB.
Carotenoid content in durian (Durio zibethinus) fruit is an important aspect of fruit quality. We analyzed fruit size, color, carotenoid composition and expression of genes involved in carotenogenesis of two durian cultivars 'Chanee' and 'Monthong' with differences in pigmentation during growth and ripening. The ripe fruit of 'Monthong' has a greater diameter and fruit weight than that of 'Chanee', while total carotenoid concentration was approximately 6-fold higher in 'Chanee' than in 'Monthong'. The major carotenoids in the fruit pulp were β-carotene and α-carotene, with minor carotenoids being lutein and zeaxanthin. Significant differences in α-carotene and β-carotene levels were seen between the two cultivars. We cloned fragments of PSY, PDS, ZDS, CRTISO, LCYB, LCYE, CYCB, BCH, ECH and Or genes from durian. Expression analysis of the carotenoid biosynthetic genes using qPCR showed that ZDS expression positively correlated with increases in total carotenoids, β-carotene, α-carotene, and lutein content and LCYE expression correlated with total carotenoids and β-carotene content. In contrast, LCYB showed a negative correlation with total carotenoids, β-carotene, α-carotene and lutein. It can be concluded that carotenoid accumulation during durian fruit growth is regulated by the balanced expression of the ZDS, LCYE and LCYB genes.
Journal: Scientia Horticulturae - Volume 220, 16 June 2017, Pages 233-242