کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5769630 | 1628778 | 2017 | 8 صفحه PDF | دانلود رایگان |
- Mdoryco1-1 of apple copia-like retrotransposon is associated with spur mutation.
- Mdoryco1-1 is a bidirectionally transcriptional retrotransposon.
- Transcriptional activity of Mdoryco1-1 was increased by ABA, wounding and 2,4-D.
- Mdoryco1element is adjacent to genes in apple genome.
Most retrotransposons from plants are believed to be either transcriptionally inactive or transcriptionally silent in somatic tissues but active during particular stages of development or under certain conditions. We have identified a bidirectionally transcriptional apple Ty1-copia-like retrotransposon called Mdoryco1-1, which was associated with apple spur mutation. Mdoryco1-1 belongs to a low-copy-number family in the sequenced apple genome. These copies were highly similar. The sense and antisense strands of the 5â²-LTR (long terminal repeat) and 3â²-LTR drove expression of GUS in transiently transformed tobacco leaves, activity which was dependent on the presence of the promoter function in the Mdoryco1-1 LTR. The transcriptional activity of Mdoryco1-1 was increased after ABA (abscisic acid), wounding and 2,4-D (2,4-dichlorophenoxyacetic acid) treatments, but not SA (salicylic acid) treatment, despite the predicted TCA-element (cis-acting element involved in salicylic acid responsiveness) in the Mdoryco1-1 LTR sense strand. The analysis of flanking sequences indicates that the Mdoryco1element is adjacent to genes in apple genome. The results suggested that the expression of Mdoryco1 could affect adjacent gene transcription.
Journal: Scientia Horticulturae - Volume 220, 16 June 2017, Pages 283-290