کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5769656 | 1628775 | 2017 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Seed-specific expression of an acyl-acyl carrier protein thioesterase CnFatB3 from coconut (Cocos nucifera L.) increases the accumulation of medium-chain fatty acids in transgenic Arabidopsis seeds
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش باغداری
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Coconut (Cocos nucifers L.) can accumulate up to nearly 80% of medium-chain fatty acids (MCFAs) in its endosperm. A previous study about suppression subtractive hybridization (SSH) libraries of coconut endosperm indicated that only one acyl-acyl carrier protein (ACP) thioesterase CnFatB3 was identified and significantly up-regulated during fruit development. Overexpression of CnFatB3 cDNA in Arabidopsis under control of the seed-specific napin promoter increased the amounts of 12:0 (lauric acid), 14:0 (myristic acid), 16:0 (palmitic acid) and 18:0 (stearic acid) by 30, 80, 4, and 2-fold, respectively, although CnFatB3 transcript levels varied 4000-fold in Arabidopsis seeds from different transgenic lines. These data suggested that CnFatB3 had a preference for 12:0, 14:0, 16:0 and 18:0-ACPs in plant. The results provide new insights into the accumulation of MCFAs in higher plants, and will contribute to the metabolic engineering of MCFAs-producing to meet energy demands.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Scientia Horticulturae - Volume 223, 15 September 2017, Pages 5-9
Journal: Scientia Horticulturae - Volume 223, 15 September 2017, Pages 5-9
نویسندگان
Yijun Yuan, Lingchao Gao, Ruhao Sun, Tian Yu, Yuanxue Liang, Dongdong Li, Yusheng Zheng,