کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5796217 | 1110689 | 2013 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Wool-associated proteolytic bacteria, isolated from Portuguese Merino breed
ترجمه فارسی عنوان
باکتری پروتئولیتیک مرتبط با پشم، جدا شده از نژاد مروینو پرتغالی
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کلمات کلیدی
گوسفند، پشم پشمی، میکرو فلور پشم، کراتین، فعالیت کازئینولیتیک،
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
چکیده انگلیسی
The main purpose of this study was to isolate and briefly characterize proteolytic bacteria from a poorly known habitat - raw wool. Fleece samples were accordingly collected from Merino raw wool - a Portuguese ewe breed, at three distinct areas of their body, from animals exhibiting no symptoms or signs of abnormalities; they were then subjected to enumeration and isolation of a total of 158 bacterial strains. Said isolates were screened for protease activity, using the spot technique, on Calcium Caseinate Agar containing 1% (w/v) skim milk. The 36 isolates displaying the highest protease activity underwent a more refined assessment of enzymatic performance - by examining their cell-free supernatant extracts, using casein as substrate. Two Bacillus isolates were eventually chosen owing to their highest proteolytic activities (24.6 and 15.9Â U/mL), and identified using molecular biology tools.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Small Ruminant Research - Volume 109, Issue 1, January 2013, Pages 38-46
Journal: Small Ruminant Research - Volume 109, Issue 1, January 2013, Pages 38-46
نویسندگان
A. Catarina Queiroga, Manuela E. Pintado, F. Xavier Malcata,