کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5796612 | 1555013 | 2016 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Recombinant Mycoplasma mycoides proteins elicit protective immune responses against contagious bovine pleuropneumonia
ترجمه فارسی عنوان
پروتئین های مایکوپلاسمی مایکوئید بازسازی شده پاسخ های محافظتی ایمنی را در برابر ملتهب مسری گاو
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
چکیده انگلیسی
Mycoplasma mycoides subsp. mycoides (Mmm) is the causative agent of contagious bovine pleuropneumonia (CBPP), a devastating respiratory disease mainly affecting cattle in sub-Saharan Africa. The current vaccines are based on live-attenuated Mmm strains and present problems with temperature stability, duration of immunity and adverse reactions, thus new vaccines are needed to overcome these issues. We used a reverse vaccinology approach to identify 66 Mmm potential vaccine candidates. The selection and grouping of the antigens was based on the presence of specific antibodies in sera from CBPP-positive animals. The antigens were used to immunize male Boran cattle (Bos indicus) followed by a challenge with the Mmm strain Afadé. Two of the groups immunized with five proteins each showed protection after the Mmm challenge (Groups A and C; PÂ <Â 0.05) and in one group (Group C) Mmm could not be cultured from lung specimens. A third group (Group N) showed a reduced number of animals with lesions and the cultures for Mmm were also negative. While immunization with some of the antigens conferred protection, others may have increased immune-related pathology. This is the first report that Mmm recombinant proteins have been successfully used to formulate a prototype vaccine and these results pave the way for the development of a novel commercial vaccine.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Immunology and Immunopathology - Volume 171, March 2016, Pages 103-114
Journal: Veterinary Immunology and Immunopathology - Volume 171, March 2016, Pages 103-114
نویسندگان
Isabel Nkando, Jose Perez-Casal, Martin Mwirigi, Tracy Prysliak, Hugh Townsend, Emil Berberov, Joseph Kuria, John Mugambi, Reuben Soi, Anne Liljander, Joerg Jores, Volker Gerdts, Andrew Potter, Jan Naessens, Hezron Wesonga,