کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5797204 1555114 2007 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sequence and phylogenetic analysis of interleukin (IL)-1β-encoding genes of five avian species and structural and functional homology among these IL-1β proteins
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Sequence and phylogenetic analysis of interleukin (IL)-1β-encoding genes of five avian species and structural and functional homology among these IL-1β proteins
چکیده انگلیسی

Interleukin (IL)-1β-encoding regions of chicken, duck, goose, turkey and pigeon were cloned and sequenced. Each IL-1β-encoding region of chicken, duck, goose and turkey is 804 nucleotides long and encodes IL-1β protein that is 268 amino acids. Pigeon IL-1β-encoding region is 810 nucleotides long and encodes IL-1β protein that is 270 amino acids. Two one-nucleotide and one four-nucleotide insertions of pigeon IL-1β-encoding region sequence were found, resulting in two amino acid insertions in pigeon IL-1β. Pairwise sequence analysis showed that the sequence identities of IL-1β-encoding genes ranged from 77% to 99%, which were also found for IL-1β protein sequence identities, with an average level of both sequence identities of 89%. Phylogenetic analysis indicated that IL-1β-encoding regions and the encoded proteins of chicken, duck, goose and turkey clustered together and evolved into a distinct phylogenetic lineage from that of pigeon which evolved into a second lineage. The results from the binding reaction of antiserum against each recombinant IL-1β (r IL-1β) protein to homologous or heterologous rIL-1β, the enhancement levels of K60 mRNA expression in rIL-1β-treated DF-1 cells or the reduction levels of K60 mRNA expression in DF-1 cells treated with rIL-1β that was preincubated with homologous or heterologous antiserum showed that all five rIL-1β were functional active and shared significantly structural and functional homology.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Immunology and Immunopathology - Volume 116, Issues 1–2, 15 March 2007, Pages 37-46
نویسندگان
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