کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5799319 1111792 2012 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Detection of Actinobacillus pleuropneumoniae in pigs by real-time quantitative PCR for the apxIVA gene
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Detection of Actinobacillus pleuropneumoniae in pigs by real-time quantitative PCR for the apxIVA gene
چکیده انگلیسی

A real-time quantitative PCR (qPCR) for detection of the apxIVA gene of Actinobacillus pleuropneumoniae was validated using pure cultures of A. pleuropneumoniae and tonsillar and nasal swabs from experimentally inoculated Caesarean-derived/colostrum-deprived piglets and naturally infected conventional pigs. The analytical sensitivity was 5 colony forming units/reaction. In comparison with selective bacterial examination using tonsillar samples from inoculated animals, the diagnostic sensitivity of the qPCR was 0.98 and the diagnostic specificity was 1.0. The qPCR showed consistent results in repeatedly sampled conventional pigs. Tonsillar brush samples and apxIVA qPCR analysis may be useful for further epidemiological studies and monitoring for A. pleuropneumoniae.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Veterinary Journal - Volume 193, Issue 2, August 2012, Pages 557-560
نویسندگان
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