کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5810142 1556216 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Microneedle-mediated transdermal bacteriophage delivery
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی اکتشاف دارویی
پیش نمایش صفحه اول مقاله
Microneedle-mediated transdermal bacteriophage delivery
چکیده انگلیسی

Interest in bacteriophages as therapeutic agents has recently been reawakened. Parenteral delivery is the most routinely-employed method of administration. However, injection of phages has numerous disadvantages, such as the requirement of a health professional for administration and the possibility of cross-contamination. Transdermal delivery offers one potential means of overcoming many of these problems. The present study utilized a novel poly (carbonate) (PC) hollow microneedle (MN) device for the transdermal delivery of Escherichia coli-specific T4 bacteriophages both in vitro and in vivo. MN successfully achieved bacteriophage delivery in vitro across dermatomed and full thickness skin. A concentration of 2.67 × 106 PFU/ml (plaque forming units per ml) was detected in the receiver compartment when delivered across dermatomed skin and 4.0 × 103 PFU/ml was detected in the receiver compartment when delivered across full thickness skin. An in vivo study resulted in 4.13 × 103 PFU/ml being detected in blood 30 min following initial MN-mediated phage administration. Clearance occurred rapidly, with phages being completely cleared from the systemic circulation within 24 h, which was expected in the absence of infection. We have shown here that MN-mediated delivery allows successful systemic phage absorption. Accordingly, bacteriophage-based therapeutics may now have an alternative route for systemic delivery. Once fully-investigated, this could lead to more widespread investigation of these interesting therapeutic viruses.

Images of PC hollow MN arrays using a variety of microscopy techniques (a) Digital microscope images of single MN and a section of the MN array (b) SEM images of a single MN, the bore of the MN and a section of the MN array (c) X-ray microtomography images of single MNs, the bore of the MN and the Mn array (d) He-ion images of a single MN, illustrating the bore of the needle and a radial view of the MN.70

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmaceutical Sciences - Volume 47, Issue 2, 29 September 2012, Pages 297-304
نویسندگان
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