کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5816474 1116135 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Inhibition of porcine detrusor contractility by the flavonoid fraction of Bryophyllum pinnatum - a potential phytotherapeutic drug for the treatment of the overactive bladder syndrome
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی بالینی
پیش نمایش صفحه اول مقاله
Inhibition of porcine detrusor contractility by the flavonoid fraction of Bryophyllum pinnatum - a potential phytotherapeutic drug for the treatment of the overactive bladder syndrome
چکیده انگلیسی

Aims: To determine if the phytotherapeutic agent, Bryophyllum pinnatum, could serve as an alternative drug for the overactive bladder syndrome, and to characterise the fraction responsible for the inhibition of detrusor contractility.Methods: Fractions were prepared from the MeOH extract of B. pinnatum and further analysed by HPLC-PDA-MS. Detrusor muscle strips were prepared from porcine bladders and the electrically induced muscle contractility measured by organ bath. The effect of B. pinnatum leaf press juice (2.5-10%), a flavonoid fraction (0.1-1 mg/ml), and a bufadienolide fraction (0.1-40 μg/ml) on detrusor contractility was assessed and compared with controls (polar fraction (0.5-5 mg/ml) and oxybutynin (10−8-10−6 M)).Results: The press juice, at a concentration of 10% led to a reduction of detrusor contractility. Bladder strips treated with the flavonoid fraction showed a significant reduction of the contractility to 21.3 ± 5.2% (1 mg/ml) while the bufadienolide fraction had no inhibitory effect in the investigated concentrations. The polar fraction showed a reduction of the contractility in a pH-dependent fashion. At 10−6 M concentration oxybutynin reduced the detrusor contractility to 21.9 ± 4.7%.Conclusions: The flavonoid fraction of Bryophyllum pinnatum reduces the porcine detrusor contractility in a dose- and time-dependent manner. Fractions from B. pinnatum may be a new pharmacological approach for the treatment of OAB.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Phytomedicine - Volume 22, Issue 1, 15 January 2015, Pages 158-164
نویسندگان
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