کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5816751 1116157 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Protective effects of propolis on female rats' histopathological, biochemical and genotoxic changes during LPS induced endotoxemia
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی بالینی
پیش نمایش صفحه اول مقاله
Protective effects of propolis on female rats' histopathological, biochemical and genotoxic changes during LPS induced endotoxemia
چکیده انگلیسی

In recent years, propolis has been the object of extensive research for its antibacterial, antioxidant, anti-inflammatory, and antitumoral activities. This study aims to determine the hepatoprotective efficiency of propolis on experimental endotoxemia in rats.In the current study, fifty adult Sprague Dawley rats (weighing 200-300 g) were randomly divided into five groups of ten rats each. Normal saline solution was administered to the rats in the control group, while in the second group LPS (30 mg/kg), in the third group propolis (250 mg/kg), in the fourth group first propolis and then LPS (30 mg/kg), and in the fifth group, first LPS (30 mg/kg) and then propolis were given. Six hours after the application, biochemical (MDA levels) and histopathological changes as well as global DNA methylation analysis in the liver tissue samples were determined, while in the blood tissue samples Genomic Template Stability (GTS, %) was evaluated using RAPD-PCR profiles.The results demonstrated that the administration of propolis could have a protective effect against changes of both genomic stability values and methylation profiles, and it minimized the increase in MDA and tissue damage caused by LPS.In conclusion, the application of propolis prior to LPS-induced endotoxemia has shown to reduce hepatic damage.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Phytomedicine - Volume 20, Issue 7, 15 May 2013, Pages 632-639
نویسندگان
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