کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5827342 | 1558923 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Elements in support of the 'non-identity' of the PGRMC1 protein with the Ï2 receptor
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
PEIPBSDMEMMCF7G418PGRMC13-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyltetrazolium bromideDulbecco׳s modified Eagle׳s medium - Dilbecco's modified Eagle's mediumMTT - MTTROS - ROSprogesterone receptor membrane component 1 - جزء غشای گیرنده پروژسترون 1human breast adenocarcinoma cells - سلول های آدنوکارسینوما سینه انسانPhosphate buffered saline - فسفات بافر شورPolyethylenimine - پلی اتیلنGeneticin - ژنتیکReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Ï2 Receptor subtype is overexpressed in a variety of human tumors, with Ï2 agonists showing antiproliferative effects towards tumor cells through multiple pathways that depend both on the tumor cell type and on the molecule type. Therefore, Ï2 receptor is an intriguing target for tumor diagnosis and treatment despite the fact that that it has not yet been cloned. One of the last attempts to characterize Ï2 receptors led to identify it as the progesterone receptor membrane component 1 (PGRMC1). Although still controversial, such identity appears to have been accepted. We the aim of contributing to solve this controversy, in this work we stably silenced or overexpressed PGRMC1 protein in human MCF7 adenocarcinoma cells. Western blotting analyses were performed to quantify the presence of PGRMC1 protein on each of the three MCF7 cell lines variants, while scatchard analyses with radioligand were performed in order to determine the expression of the Ï2 receptors. In order to correlate the antiproliferative effect of Ï2 receptor agonist with PGRMC1 density, some Ï2 ligands were administered to each of the three MCF7 cells variants. The results suggested that PGRMC1 and Ï2 receptors are two different molecular entities.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmacology - Volume 758, 5 July 2015, Pages 16-23
Journal: European Journal of Pharmacology - Volume 758, 5 July 2015, Pages 16-23
نویسندگان
Carmen Abate, Mauro Niso, Vittoria Infantino, Alessio Menga, Francesco Berardi,