کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5828602 1558967 2013 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Pulmonary, gastrointestinal and urogenital pharmacologyNocistatin inhibits pregnant rat uterine contractions in vitro: Roles of calcitonin gene-related peptide and calcium-dependent potassium channel
ترجمه فارسی عنوان
داروهای ریه، داروهای دستگاه گوارش و مجاری ادراری، نکتستین، انقباضات رحم باردار را در شرایط آزمایشگاهی مهار می کند: نقش پپتید مرتبط با ژن کلسی تونین و کانال پتاسیم وابسته به کلسیم
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب سلولی و مولکولی
چکیده انگلیسی

The endogenous neuropeptide nociceptin/orphanin FQ, translated from the prepronociceptin gene, exerts a contraction-inhibitory effect on the rat uterus. As nocistatin has been reported to cause functional antagonism of the pro-nociceptive effects of nociceptin, we set out to investigate its effects on the pregnant rat uterus and to elucidate its signalling pathway. The expression of prepronociceptin mRNA in the uterus and nocistatin levels in the uterus and the plasma were confirmed by RT-PCR and radioimmunoassay. The uterine levels of prepronociceptin mRNA and nocistatin were significantly increased by the last day of pregnancy, while the plasma nocistatin levels remained unchanged. In the isolated organ bath studies nocistatin inhibited the prostaglandin- and the KCl-evoked contractions in the uterus dose-dependently. This latter effect was decreased by preincubation with capsaicin. Incubation with calcitonin gene-related peptide after capsaicin treatment caused an elevation in the contraction-inhibitory effect of nocistatin. The effect of nocistatin was also decreased by the Ca2+-dependent K+ channel inhibitor paxilline, against spontaneous uterine contractions. Nociceptin potentiated the action of nocistatin. Naloxone decreased the effect of nocistatin administered either alone or in combination with nociceptin. In Ca2+-poor environment, this effect of naloxone was suspended. Enzyme immunoassay for the uterine intracellular cAMP levels partially confirmed the results of in vitro contractility studies. We conclude that nocistatin, generated locally in the uterus, exerts an inhibitory effect, the mechanism being mediated in part by Ca2+-dependent K+ channels, the elevation of cAMP levels and sensory neuropeptides.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmacology - Volume 714, Issues 1–3, 15 August 2013, Pages 96-104
نویسندگان
, , , , , ,