Isolation, characterization and acetylcholinesterase inhibitory activity of alkaloids from roots of Stemona sessilifolia

Two new alkaloids, named stenine A (1) and stenine B (2), along with the known compounds neostenine (3), stenine (4) and neotuberostemonine (5), were isolated from the roots of Stemona sessilifolia. Their structures were elucidated by 1D- and 2D-NMR spectra and X-ray single-crystal diffraction experiment. Anti-acetylcholinesterase (AChE) activity of compounds 1–5 were also tested. Compounds 2 and 4 showed significant anti-acetylcholinesterase activities, with IC50 values of 2.1 ± 0.2 μM and 19.8 ± 2.5 μM, resp. The mode of AChE inhibition by Compound 2 (the most potential AChE inhibitor) was reversible and competitive. In addition, molecular modeling was performed to explore the binding mode of compound 2 with AChE.

Two new alkaloids, stenine A (1) and stenine B (2) were isolated and identified from Stemona sessilifolia. Their acetylcholinesterase inhibitory activity was estimated. Compound 2 showed significant anti-acetylcholinesterase activities, with IC50 values of 2.1 ± 0.2 μM.Figure optionsDownload high-quality image (120 K)Download as PowerPoint slide