کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5849430 | 1561756 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Sodium benzoate, a food preservative, affects the functional and activation status of splenocytes at non cytotoxic dose
ترجمه فارسی عنوان
بنزوات سدیم، یک نگهدارنده غذا، بر وضعیت عملکرد و فعال سازی اسپلنوسیت ها در دوز غیر سیتوتوکسیک تاثیر می گذارد
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کلمات کلیدی
TNFAPCAPC-Cy7MLRConcanavalin AFITCPerCPDMEMLPSDulbecco's modified Eagles medium - Medal of Eagles اصلاح شده Dulbeccoallophycocyanin - آلوفوکسیانینSplenocytes - اسپلنوسیت هاinterferon - اینترفرونIFN - اینترفرون هاinterleukin - اینترلوکینCon A - با ASodium benzoate - بنزوات سدیمClusters of Differentiation - خوشه های تمایزImmunotoxicity - سمیت ایمنیCytokines - سیتوکین هاtumor necrosis factor - فاکتور نکروز تومورphycoerythrin - فایکوئیریدینfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتLymphoproliferation - لنفوپرولیفراسیونlipopolysaccharide - لیپوپلی ساکاریدActivation markers - نشانگر فعال سازیmixed lymphocyte reaction - واکنش لنفوسیت مخلوطperidinin chlorophyll protein - پریدین پروتئین کلروفیل
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
چکیده انگلیسی
Sodium benzoate (SB) is a widely used food preservative due to its bacteriostatic and fungistatic properties. The acceptable daily intake of SB is 5 mg/kg-bw, however, it has been found to be used in the food commodities at relatively high levels (2119 mg/kg). Earlier studies on SB have shown its immunosuppressive properties, but comprehensive immunotoxicity data is lacking. Our studies have shown that SB was non cytotoxic in splenocytes up to 1000 μg/ml for 72 h, however at 2500 μg/ml it was found to be cytotoxic. Thus, 1000 μg/ml dose of SB was chosen for the subsequent experiments. SB significantly suppresses the proliferation of Con A and LPS stimulated splenocytes at 72 h, while allogenic response of T cells was significantly decreased after 96 h. SB did not affect the relative expression of CD3e or CD4 molecules following 72 h exposure, however, it downregulated the relative expression of CD8 co-receptor. Further, exposure of splenocytes to SB for 72 h led to reduced expression of CD28 and CD95, which play a vital role in T cell activation. SB also suppresses the relative expression of CD19, CD40 and CD95 receptors on B cells after 72 h. In addition to the functional responses, SB lowered the expression of IL4, IL6, IFNγ and IL17 cytokines in Con A stimulated splenocytes; and IL6, IFNγ and TNFα in LPS stimulated splenocytes following 48 h of exposure. Taken together, the present study is suggestive of the immunomodulatory potential of SB.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 88, February 2016, Pages 40-47
Journal: Food and Chemical Toxicology - Volume 88, February 2016, Pages 40-47
نویسندگان
Ashish Yadav, Arvind Kumar, Mukul Das, Anurag Tripathi,