کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5850819 1561781 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Chloroform fraction of Solanum tuberosum L. cv Jayoung epidermis suppresses LPS-induced inflammatory responses in macrophages and DSS-induced colitis in mice
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Chloroform fraction of Solanum tuberosum L. cv Jayoung epidermis suppresses LPS-induced inflammatory responses in macrophages and DSS-induced colitis in mice
چکیده انگلیسی


- CFPJ is chloroform fraction isolated from peel of 'Jayoung', a color-fleshed potato.
- CFPJ inhibited the LPS-induced iNOS, COX-2, and cytokine expressions in macrophages.
- CFPJ suppressed the LPS-induced activation of NF-κB/IκB-α and p38/MKK signaling.
- CFPJ attenuated the DSS-induced colitis severity assessed by DAI and colon length.
- CFPJ suppressed the production of pro-inflammatory mediators in DSS-induced colitis.

In this study, the authors investigated the molecular mechanism underlying the antiinflammatory effects of the chloroform fraction of the peel of 'Jayoung' (CFPJ), a color-fleshed potato, on lipopolysaccharide (LPS)-induced RAW 264.7 macrophages and in mice with dextran sulfate sodium (DSS)-induced colitis. CFPJ inhibited the expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at the transcription level, and attenuated the transcriptional activity of nuclear factor-κB (NF-κB) by reducing the translocation of NF-κB depending on degradation of inhibitory κB-α (IκB-α). Furthermore, CFPJ attenuated the phosphorylations of mitogen-activated protein kinase kinases3/6 (MKK3/6) and of p38. In colitis model, CFPJ significantly reduced the severity of colitis and the productions and protein levels of pro-inflammatory mediators in colonic tissue. These results suggest that the anti-inflammatory effects of CFPJ are associated with the suppression of NF-κB and p38 activation in macrophages, and support its possible therapeutic role for the treatment of colitis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 63, January 2014, Pages 53-61
نویسندگان
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