کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5851076 | 1561785 | 2013 | 6 صفحه PDF | دانلود رایگان |
- The combination of probiotics and AFBl-degrading enzyme could degrade AFB1.
- It was able to relieve negative effect of AFB1 on chicken's production performance.
- The hepatic cell structure and antioxidant ability were improved by the combination.
- Many hepatic enzyme gene expressions were influenced by the combination.
In order to degrade aflatoxin B1 (AFB1), AFBl-degrading microbes (probiotics) such as Lactobacillus casei, Bacillus subtilis and Pichia anomala, and the AFBl-degrading enzyme from Aspergillus oryzae were selected and combined to make feed additive. Seventy-five 43-day-old male Arbor Acres broilers were randomly divided into 5 groups, 15 broilers for each group. The broilers were given with 5 kinds of diets such as the basal diet, 400 μg/kg AFB1 supplement without feed additive, and 200, 400, 800 μg/kg AFB1 supplement with 0.15% feed additive. The feeding experimental period was 30 d, which was used to determine production performance of broilers. In addition, serum, liver and chest muscle were selected for measuring AFB1 residues, gene expressions, microscopic and antioxidant analyses. The results showed that adding 0.15% feed additive in broiler diets could significantly relieve the negative effect of AFB1 on chicken's production performance and nutrient metabolic rates (P < 0.05). It could also improve AFB1 metabolism, hepatic cell structure, antioxidant activity, and many hepatic enzyme gene expressions involved in oxidoreductase, apoptosis, cell growth, immune system and metabolic process (P < 0.05). It could be concluded that the feed additive was able to degrade AFB1 and improve animal production.
Journal: Food and Chemical Toxicology - Volume 59, September 2013, Pages 470-475