کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5851120 | 1561785 | 2013 | 8 صفحه PDF | دانلود رایگان |

- To investigate I. suffruticosa extracts on expression of GSTP and NQO1 in hepatocytes.
- Suffruticosa extracts increased GSTP and NQO1 levels and GSTP promoter activity in hepatocyte.
- Suffruticosa extracts up-regulated binding affinity of Nrf2 with GPEI and ARE activation.
- ERK inhibitor abolished I. suffruticosa extracts induced GSTP and NQO-1 expression.
- Suffruticosa extracts increased GSH levels in mouse liver, RBC and hepatocytes.
Because induction of phase II detoxification enzyme is important for chemoprevention, we study the effects of Indigofera suffruticosa Mill, a medicinal herb, on the expression of Ï class of glutathione S-transferase (GSTP) and NAD(P)H: quinone oxidoreductase 1 (NQO1) in rat Clone 9 liver cells. Both water and ethanolic extracts of I. suffruticosa significantly increased the expression and enzyme activities of GSTP and NQO1. I. suffruticosa extracts up-regulated GSTP promoter activity and the binding affinity of nuclear factor erythroid 2-related factor 2 (Nrf2) with the GSTP enhancer I oligonucleotide. Moreover, I. suffruticosa extracts increased nuclear Nrf2 accumulation as well as ARE transcriptional activity. The level of phospho-ERK was augmented by I. suffruticosa extracts, and the ERK inhibitor PD98059 abolished the I. suffruticosa extract-induced ERK activation and GSTP and NQO-1 expression. Moreover, I. suffruticosa extracts, especially the ethanolic extract increased the glutathione level in mouse liver and red blood cells as well as Clone 9 liver cells. The efficacy of I. suffruticosa extracts in induction of phase II detoxification enzymes and glutathione content implies that I. suffruticosa could be considered as a potential chemopreventive agent.
Journal: Food and Chemical Toxicology - Volume 59, September 2013, Pages 610-617