Short-term assessment of toxicological aspects, oxidative and inflammatory response to dietary melon superoxide dismutase in rats

The protective effects of SODB, a gastro-resistant encapsulated melon superoxide dismutase, on haematological and biochemical parameters and inflammatory and oxidative status, were evaluated in the blood and liver tissue. The study consisted in a 28-day experiment on rats supplemented with three doses (10, 40 and 160 U SOD/day) of SODB-M, SODB-D or SODB-S, different depending on the nature of the coating (palm oil, shellac or gum Arabic respectively). No mortality, abnormal clinical signs, behavioural changes or macroscopic findings were observed whatever the groups. Haematological parameters (total red blood cell count, haemoglobin content, haematocrit, red cell indices, white blood cell count and platelets count) were not modified in SODB treated-groups. No marked change was recorded in biochemical parameters (plasma urea, creatinine, lipids, electrolytes, bilirubin, transaminases and gamma-glutamyl transferase). The liver endogenous antioxidant enzymes (copper/zinc and manganese superoxide dismutase) expressions were significantly increased in the rats receiving the highest dose of SODB (160 U SOD/day) whatever the coating. Moreover, interleukin-6, a marker of inflammation, was significantly decreased in these high dose-treated-groups.The present study indicates that dietary supplementation of SODB on rats has no harmful side effects and could be beneficial especially at high doses.

► SODB supplementation does not induce any toxicity. ► SODB supplementation (at the dose of 160 U SOD/day for 28 days) induces an increase in hepatic endogenous SODs. ► SODB supplementation (at the dose of 160 U SOD/day for 28 days) induces a decrease in hepatic interleukin-6.