کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5852077 1561787 2013 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cytotoxic mechanism of Piper gaudichaudianum Kunth essential oil and its major compound nerolidol
ترجمه فارسی عنوان
مکانیسم سیتوتوکسیک روغن اسانس کیک پپور گودوخودینوم و ترکیب اصلی آن نئیدیلول
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
چکیده انگلیسی


- Nerolidol is a sesquiterpene present in the essential oil of Piper gaudichaudianum.
- Essential oil and nerolidol led to cytotoxicity in XV185-14c and N123 strains.
- BER defective strains showed pronounced sensitivity to essential oil and nerolidol.
- Superoxide dismutase absence increases the sensitivity to the essential oil and nerolidol.
- P. gaudichaudianum essential oil and nerolidol induce the ROS production.

Piper gaudichaudianum Kunth is used in popular medicine as anti-inflamatory and against liver disorders. One of the most studied components of the plant is the essential oil for which chemical analysis revealed (E)-nerolidol as major compound. Recently, we have shown that P. gaudichaudianum essential oil possesses strong cytotoxic effects in mammalian V79 cells. The aim of this study was to analyze the cytotoxicity and mutagenicity of P. gaudichaudianum essential oil and nerolidol using Saccharomyces cerevisiae as model study. Treatment of the XV185-14c and N123 strains with essential oil and nerolidol led to cytotoxicity but did not induce mutagenicity. Our results revealed an important role of base excision repair (BER) as the ntg1, ntg2, apn1 and apn2 mutants showed pronounced sensitivity to essential oil and nerolidol. In the absence of superoxide dismutase (in sod1Δ mutant strain) sensitivity to the essential oil and nerolidol increased indicating that this oil and nerolidol are generating reactive oxygen species (ROS). The ROS production was confirmed by DCF-DA probing assay in Sod-deficient strains. From this, we conclude that the observed cytotoxicity to P. gaudichaudianum essential oil and nerolidol is mainly related to ROS and DNA single strand breaks generated by the presence of oxidative lesions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 57, July 2013, Pages 57-68
نویسندگان
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