Synthesis of hypoallergenic derivatives of the major allergen Fag t 1 from tartary buckwheat via sequence restructuring

► Three hypoallergenic Fag t 1 derivatives were generated via a sequence reassembly. ► The structural integrity and stability of the Fag t 1 derivatives were analyzed. ► The destruction of the native conformation may be the appropriate strategy for allergen-specific immunotherapy (SIT). ► The method to reduce the allergenicity of the cupin family food allergens.