Toxicity and carcinogenicity of androstenedione in F344/N rats and B6C3F1 mice

► We investigated the chronic toxicity and carcinogenicity activity of androstenedione. ► Androstenedione may or may not carcinogenic in F344/N rats. ► Androstenedione was carcinogenic in B6C3F1 mice. ► Androstenedione decreased tumors in endocrine sensitive reproductive organs.