The rapid establishment and implications of a melamine-induced standardized bladder stone model in mice

► The key to establishing a melamine cystolith model is to determine its dose in diet. ► The golden section (0.618) of mouse oral melamine LD50 as an average daily dose. ► The daily dose corresponds to 9373 ppm melamine diet. ► The 9373 ppm melamine diet is ideal for creating the model in Balb/c or C57BL/6 mice. ► Dietary melamine dose or intake neither sex nor age is critical for stone formation.