Evaluation of the developmental toxicity of leucomalachite green administered orally to rats

► The unmetabolized compound was detected in the plasma of the rat treated with LMG. ► LMG entered the amniotic fluid through the placental barrier. ► LMG at 160 mg/kg/day caused embryolethality in rats. ► LMG at 80-160 mg/kg/day caused fetal developmental toxicity in rats. ► In this study, the maternal and developmental toxicity NOAEL of LMG was 10 mg/kg/day.