Analysis of combined effects of nonylphenol and Monobutyl phthalate on rat Sertoli cells applying two mathematical models

► Two mathematical models according to LA theory and BI theory were applied analyze the combined effect of NP and MBP. ► Employing cell viability and lactate dehydrogenase (LDH) leakage rate as endpoints. ► A significant antagonist effect was observed both in the viability assay and in the LDH assay (LDH leakage rate >20%). ► At the lower concentrations which induced LDH leakage rate <20%, the combined effect was synergism.