کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5853725 1130863 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
1H NMR study of monocrotaline and its metabolites in human blood
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
1H NMR study of monocrotaline and its metabolites in human blood
چکیده انگلیسی

Monocrotaline (MCT) is a naturally occurring hepatotoxic pyrrolizidine alkaloid found in plants. This investigation is aimed at furthering the understanding of the role of blood in mediating the transport of MCT and its reactive metabolites in humans. Reactions of monocrotaline and its metabolites, dehydromonocrotaline (DHM), retronecine (RET) and dehydroretronecine (DHR) with human blood plasma, red blood cells (RBCs), and whole blood were studied in vitro by proton nuclear magnetic resonance spectroscopy. In plasma MCT remained intact and weakly associated with plasma proteins, and DHM was rapidly hydrolyzed releasing necic and lactone acids, and the reactive pyrrolic metabolite. MCT and its metabolite DHM were internalized in RBCs to the extent of 46.0% and 48.9% respectively in 30 min. No polymerization of DHR was observed when incubated with plasma and RBCs. The data clearly showed that both human plasma and RBCs could be the carriers for the transportation of MCT and its metabolites, DHM, RET and DHR between organs and could stabilise the reactive MCT metabolite DHR.

► Both human plasma and red blood cells (RBCs) can transport monocrotaline (MCT) and its metabolites. ► MCT (46%) and its toxic metabolite dehydromonocrotaline (DHM, 49%) were taken into RBCs in 30 min. ► In plasma MCT remained intact and weakly associated with plasma proteins. ► DHM was rapidly hydrolyzed to a reactive pyrrolic metabolite in plasma.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 49, Issue 11, November 2011, Pages 2793-2799
نویسندگان
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