کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5874076 | 1144665 | 2014 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Alteration in Bioenergetic Regulators, SirT1 and Parp1 Expression Precedes Oxidative Stress in Rats Subjected to Transient Cerebral Focal Ischemia: Molecular and Histopathologic Evidences
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
مغز و اعصاب بالینی
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چکیده انگلیسی
The present study was undertaken to identify the time-dependent changes in bioenergetic regulators and oxidative stress markers levels and also to ascertain which occurs early in focal cerebral ischemia. The status of bioenergetic regulators (nicotinamide adenine dinucleotide, Silent information regulator Transporter 1 (SirT1) and Poly adenosine diphosphate - ribose polymerase 1 (Parp1)) and oxidative stress markers' (lipid peroxidation and nitric oxide) levels along with excitatory neurochemical glutamate, neuronal nuclei (NeuN), glial fibrillary acidic protein (GFAP), and caspase 3 at various durations of reperfusion viz 0, 1, 6, 24, 72, and 166Â hours was investigated in rats subjected to 2Â hours of middle cerebral artery occlusion. Six hours after reperfusion, a significant downregulation in SirT1 and upregulation in Parp1 expressions were observed in striatal region of ischemic-reperfused rats compared with ischemic rats. Peak elevation in glutamate content was recorded at 72Â hours, and maximum level of lipid peroxidation and nitric oxide contents were observed at 166Â hours. A significant decrease in NeuN and increase in GFAP-positive cells were observed at 24 and 72Â hours, respectively, in cortical, striatal, and hippocampal regions. These data reveal that change in bioenergetic regulators occurs earlier than excitotoxicity and oxidative stress in cerebral stroke. From the data, it can also be inferred that appropriate therapeutic intervention, that is, bioenergetic modulators at early period and either glutamatergic and/or antioxidant therapy at delayed period may yield a better therapeutic outcome.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Stroke and Cerebrovascular Diseases - Volume 23, Issue 10, NovemberâDecember 2014, Pages 2753-2766
Journal: Journal of Stroke and Cerebrovascular Diseases - Volume 23, Issue 10, NovemberâDecember 2014, Pages 2753-2766
نویسندگان
Periyathambi MSc, MPhil, Murugan MBBS, MD, Sekar MSc, Vijayan MSc, Veeraragavan MSc, PhD, Chidambaram MPharm, PhD,