کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5886962 1150946 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
SepsisEarly bacterial genome detection in body fluids from patients with severe sepsis: A pilot study★
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی بیهوشی و پزشکی درد
پیش نمایش صفحه اول مقاله
SepsisEarly bacterial genome detection in body fluids from patients with severe sepsis: A pilot study★
چکیده انگلیسی

PurposeThe purpose of this study is to evaluate the feasibility and interest of real-time polymerase chain reaction (RT-PCR) testing for bacterial genomes in body fluids other than blood in patients with acute severe sepsis.MethodsTwenty-six consecutive patients admitted for severe sepsis or septic shock were prospectively studied. Body fluids were sampled as clinically indicated and tested using standard microbiological methods and modified RT-PCR methods (universal PCR and specific PCRs). Results of standard microbiological tests were compared with those of PCR tests.ResultsDirect RT-PCR testing was successfully performed on all nonblood body fluids. Of 29 body fluids collected, 23 were positive for at least 1 microorganism with conventional tests. Of 18 microbiological tests positive for a single microorganism, 15 fully agreed with RT-PCR assays, and the remaining 3 samples were infected with bacteria not screened by PCR testing. Among the 5 polymicrobial results obtained with conventional tests, RT-PCR agreed in 4 patients. The RT-PCR tests allowed additional clinically relevant bacterial identification in 3 of 6 samples with negative microbiological culture.ConclusionsOur results indicate that direct PCR testing may improve the detection of bacteria in body fluids other than blood in patients with acute severe sepsis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Critical Care - Volume 27, Issue 4, August 2012, Pages 416.e1-416.e6
نویسندگان
, , , , , , , , ,