کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5894619 | 1154436 | 2015 | 8 صفحه PDF | دانلود رایگان |

- 3D models of the last three branching levels of villous trees were prepared.
- The samples were serially sectioned and reconstructed in 3D.
- Villous types were classified on these serial sections by morphologists.
- Inter-rater variability was determined, villous types were matched to 3D position.
- Inter-rater variability and villous types to 3D mismatch were substantial.
IntroductionThe villous tree of human placentas is a complex three-dimensional (3D) structure which enables fetomaternal exchange. Current concepts of microscopic analyses are based on the analysis of two-dimensional (2D) histologic sections. For this approach, the assessment of the stromal core of sectioned villi is of key importance. The classification of stromal properties of sectioned villi allows allocation of villous sections to villous types which are named by their expected position in villous trees (terminal, intermediate, and stem villi).MethodThe present study takes these current concepts of placental histology as hypothesis and validates them against predetermined 3D positions of branches of villous trees. The 3D positions were determined prior to histologic sectioning using a recently introduced 3D-microscopic approach. Individual histologic sections of villi were classified by their stromal structures and inter rater variability of these histologic assessments were determined.Results/disscussionInter rater variability was high and indicates substantial observer influence on the outcome of histologic assessments. Cross-match of villous types with the predetermined positions of villous branches of villous trees revealed substantial mismatch between the outcome of stromal classification and 3D-position of the sectioned villi in the placental villous trees.
Journal: Placenta - Volume 36, Issue 12, December 2015, Pages 1425-1432